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Generation of clinical-grade human embryonic stem cell line KthES11 according to Japanese regulations.

Stem cell research - 1 Jul 2021

Kawase Eihachiro, Takada Kei, Nakatani Ryoko, Yamazaki Shizuka, Suemori Hirofumi

Abstract excerpt

The human embryonic stem cell line, KthES11, is derived from a normal healthy blastocyst donated for clinical research. The inner cell mass (ICM) was isolated using mechanical dissection and plated on laminin fragments. Cell line derivation, its propagation and storage were performed without feeders in an animal product-free environment according to current Good Manufacturing Practice (cGMP) standards. KthES11...

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PMID: 34126558
DOI: 10.1016/j.scr.2021.102383

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