Article
Human papillomavirus type 16 P670 promoter is negatively regulated by CCAAT displacement protein.
Virus genes - 1 Dec 2007
Sato Kaori, Takeuchi Takamasa, Kukimoto Iwao, Mori Seiichiro, Yasugi Toshiharu, Yano Tetsu, Taketani Yuji, Kanda Tadahito
Abstract excerpt
HPV16 late gene transcription from P670 is suppressed in undifferentiated keratinocytes. To identify DNA sites involved in the negative regulation, we examined the effect of a series of substitutions in the P670 promoter region (nucleotide (nt) 106-855) on the transcription, using an expression plasmid having the promoter fragment placed to drive the firefly-luciferase gene. Twenty-base pair-long segments...
Topics
- Artificial Gene Fusion
- Binding Sites
- Cell Line
- DNA, Viral
- Electrophoretic Mobility Shift Assay
- Genes, Reporter
- Homeodomain Proteins
- Human papillomavirus 16
- Humans
- Luciferases, Firefly
- Models, Biological
- Mutation
- Nuclear Proteins