Article
[Study on the construction of standard D12S391 allelic ladder and its genetic polymorphism in six populations].
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics - 1 Feb 2002
Zhang Lin, Xin Junping, Chen Guodi, Liao Miao, Li Ronghua
Abstract excerpt
OBJECTIVE: To resolve the problem of the accuracy and standardization of short tandem repeat-polymerase chain reaction (STR-PCR) typing in forensic practice, the authors have designed a new method of producing standard D12S391 allelic ladder. METHODS: Nine different PCR amplified D12S391 allelic fragments were isolated from the gel, eluted into the distilled water and re-amplified by PCR. The purified allelic...
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