Article
A new method for isolating physiologically active Mg-protoporphyrin monomethyl ester, the substrate of the cyclase enzyme of the chlorophyll biosynthetic pathway.
Plant physiology and biochemistry : PPB - 1 Dec 2007
Gough Simon P, Rzeznicka Kamila, Peterson Wulff Ragna, Francisco Jose da Cruz, Hansson Andreas, Jensen Poul Erik, Hansson Mats
Abstract excerpt
Mg-protoporphyrin monomethyl ester (MPE) is a biosynthetic intermediate of chlorophyll and converted by MPE cyclase to protochlorophyllide. Limited availability of MPE has so far hampered cyclase research. In a new, simplified, method MPE was prepared from freeze dried bchE mutant Rhodobacter capsulatus DB575 cells by extraction with acetone/H(2)O/25% NH(3). Isolated MPE was identified by absorption and...
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