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Targeted knockout of barley Ycf54 demonstrates its essential function in the Mg-protoporphyrin IX monomethyl ester cyclase involved in chlorophyll biosynthesis.

Hereditas - 28 May 2026

Youssef Helmy M, Hoffie Iris, Nordling Otto, Stuart David, Zakhrabekova Shakhira, Helmi Radwa Y, Kumlehn Jochen, Hansson Mats

Abstract excerpt

BACKGROUND: The Mg-protoporphyrin IX monomethyl ester cyclase is one of the 15 enzymes required for biosynthesis of chlorophyll in plants. The Ycf54 protein is a component associated with this enzyme and probably functions as a chaperone. Due to the lack of mutants in the Ycf54 gene, we targeted this gene in barley (Hordeum vulgare L.) by RNA-guided Cas9 genome editing to dissect its role in chlorophyll...

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PMID: 42210318
DOI: 10.1186/s41065-026-00693-8
PMCID: PMC13221752

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