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Live rats resulting from injection of oocytes with spermatozoa freeze‐dried and stored for one year

9 Oct 2007

Abstract excerpt

This study was designed to examine whether rat spermatozoa after freeze-drying and 1-year storage can participate in full-term development following intracytoplasmic sperm injection (ICSI). Cauda epididymal spermatozoa from Crlj:Wistar rats were frozen in liquid nitrogen (LN(2)), first dried for 14 hr at 0.37 hPa and then for 3 hr at 0.001 hPa. The dried spermatozoa were stored for 1 year in a desiccator at +25...

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PMID: 17926349
DOI: 10.1002/mrd.20825

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