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Article

Production of Offspring from One-Day-Old Oocytes Stored at Room Temperature

1 Jan 2004

Abstract excerpt

To determine the feasibility of preserving oocytes without freezing, we stored mouse oocytes in several media at different temperatures for one day. Confocal microscopy of the metaphase-II spindle in these stored oocytes revealed gross abnormalities in both the spindle and the arrangement of chromosomes. The abnormal spindles could not be rescued by transplanting the aged spindle-chromosome complex into a fresh...

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PMID: 15647614
DOI: 10.1262/jrd.50.627

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