Functional analysis by site-directed mutagenesis of the NAD(+)-reducing hydrogenase from Ralstonia eutropha.

doi:10.1128/JB.184.22.6280-6288.2002

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Article

Functional analysis by site-directed mutagenesis of the NAD(+)-reducing hydrogenase from Ralstonia eutropha.

Journal of bacteriology - 1 Nov 2002

Burgdorf Tanja, De Lacey Antonio L, Friedrich Bärbel

Abstract excerpt

The tetrameric cytoplasmic [NiFe] hydrogenase (SH) of Ralstonia eutropha couples the oxidation of hydrogen to the reduction of NAD(+) under aerobic conditions. In the catalytic subunit HoxH, all six conserved motifs surrounding the [NiFe] site are present. Five of these motifs were altered by site-directed mutagenesis in order to dissect the molecular mechanism of hydrogen activation. Based on phenotypic...

Topics

Amino Acid Substitution
Binding Sites
Cupriavidus necator
Hydrogen
Hydrogenase
Mutagenesis, Site-Directed
NAD
Oxidation-Reduction
Oxidoreductases
Phenotype
Solubility

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Identifiers

PMID: 12399498
DOI: 10.1128/JB.184.22.6280-6288.2002
PMCID: PMC151951

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