Article
Real-time multiplex PCR assays for reliable detection of Clostridium perfringens toxin genes in animal isolates.
Veterinary microbiology - 5 Feb 2008
Albini S, Brodard I, Jaussi A, Wollschlaeger N, Frey J, Miserez R, Abril C
Abstract excerpt
Typing of Clostridium perfringens strains by PCR-based determination of toxin genes proved to be a reliable method for diagnosis of enterotoxaemia in various animal species. We report the establishment and validation of three real-time fluorogenic (TaqMan) multiplex PCRs for the detection of C. perfringens alpha-, beta-, beta2-, epsilon-, entero- and iota-toxin genes. The composition of the PCRs was chosen with...
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