Article
Development, validation and implementation of a quadruplex real-time PCR assay for identification of potentially toxigenic corynebacteria
1 Nov 2016
Abstract excerpt
Toxigenic corynebacteria are uncommon in the UK; however, laboratory confirmation by the national reference laboratory can inform public health action according to national guidelines. Standard phenotypic tests for identification and toxin expression of isolates can take from ≥24 to ≥48 h from receipt. To decrease the time to result, a real-time PCR (qPCR) assay was developed for confirmation of both...
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