Article
Isolation of a trifluoroleucine-resistant mutant of Saccharomyces cerevisiae deficient in both high- and low-affinity L-leucine transport.
Cellular and molecular biology (Noisy-le-Grand, France) - 1 Sept 1996
Chianelli M S, Stella C A, Sáenz D A, Ramos E H, Kotliar N, Mattoon J R
Abstract excerpt
A yeast mutant defective in permeases S1 and S2 which transport L-leucine was isolated from a parental strain already deficient in the general amino acid permease, GAP1. The mutant was selected as a spontaneous, trifluoroleucine-resistant (TFLR) strain. Full resistance depended upon the presence...
Topics
- Adenosine Triphosphatases
- Affinity Labels
- Amino Acid Transport Systems
- Biological Transport
- Drug Resistance, Microbial
- Fungal Proteins
- Leucine
- Membrane Transport Proteins
- Mutation
- Saccharomyces cerevisiae
- Schizosaccharomyces pombe Proteins