Article
Extrachromosomal recombination is deranged in the rec2 mutant of Ustilago maydis.
Genetics - 1 Dec 1991
Fotheringham S, Holloman W K
Abstract excerpt
Transformation of a leu1 auxotroph of Ustilago maydis to prototrophy with an autonomously replicating plasmid containing the selectable LEU1 gene was found to be efficient regardless of whether the transforming DNA was circular or linear. When pairs of autonomously replicating plasmids bearing noncomplementing leu1 alleles were used to cotransform strains deleted entirely for the genomic copy of the LEU1 gene,...
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