Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Sensitive detection of FGFR3 mutations in bladder cancer and urine sediments by peptide nucleic acid-mediated real-time PCR clamping.

Biochemical and biophysical research communications - 3 Nov 2007

Miyake Makito, Sugano Kokichi, Kawashima Kiyotaka, Ichikawa Hiroki, Hirabayashi Kaoru, Kodama Tetsuro, Fujimoto Hiroyuki, Kakizoe Tadao, Kanai Yae, Fujimoto Kiyohide, Hirao Yoshihiko

Abstract excerpt

Somatic mutations of the fibroblast growth factor receptor 3 (FGFR3) gene were detected by peptide nucleic acid (PNA)-mediated real-time PCR clamping. Mutation was detected in negative control containing only wild-type DNA due to a misincorporation of dNTPs to PNA binding sites when the amount of template DNA was decreased to 1 ng. Thus, the amount of template DNA was critical determinant of the assay sensitivity...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to DNA Mutational Analysis
Share to DNA Probes

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Peptide Nucleic Acids

Share to Receptor, Fibroblast Growth Factor, Type 3

Share to Reverse Transcriptase Polymerase Chain Reaction

Share to Sensitivity and Specificity

Share to Urinalysis

Share to Urinary Bladder Neoplasms

Source

Identifiers

PMID: 17803960
DOI: 10.1016/j.bbrc.2007.08.092

Genes

FGFR3

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Sensitive detection of FGFR3 mutations in bladder cancer and urine sediments by peptide nucleic acid-mediated real-time PCR clamping.PMID 17803960Read ↗

Detection of low frequency FGFR3 mutations in the urine of bladder cancer patients using next-generation deep sequencingSemantic proximity Read ↗

A simple and fast method for the simultaneous detection of nine fibroblast growth factor receptor 3 mutations in bladder cancer and voided urine.Semantic proximity Read ↗

Sensitive allele-specific PCR assay able to detect FGFR3 mutations in tumors and urine from patients with urothelial cell carcinoma of the bladder.Semantic proximity Read ↗

A novel ultra-sensitive method for the detection of FGFR3 mutations in urine of bladder cancer patients – Design of the Urodiag® PCR Kit for surveillance of patients with non-muscle-invasive bladder cancer (NMIBC)Semantic proximity Read ↗

A novel ultra-sensitive method for the detection of FGFR3 mutations in urine of bladder cancer patients - Design of the Urodiag® PCR kit for surveillance of patients with non-muscle-invasive bladder cancer (NMIBC).Semantic proximity Read ↗

Usefulness of droplet digital PCR and Sanger sequencing for detection of FGFR3 mutation in bladder cancerSemantic proximity Read ↗

Identification of fibroblast growth factor receptor 3 mutations in urine sediment DNA samples complements cytology in bladder tumor detectionSemantic proximity Read ↗

Fibroblast growth factor receptor 3 gene (FGFR3) mutations in high-grade muscle-invasive urothelial bladder cancer in a Brazilian population: evaluation and prevalence.Semantic proximity Read ↗

Fibroblast growth factor receptor 3 mutation in voided urine is a useful diagnostic marker and significant indicator of tumor recurrence in non-muscle invasive bladder cancer.Semantic proximity Read ↗

Urinary cell-free DNA as a potential tumor marker for bladder cancerSemantic proximity Read ↗

Urinary Cell-Free DNA as a Potential Tumor Marker for Bladder CancerSemantic proximity Read ↗

Optimization of nonmuscle invasive bladder cancer recurrence detection using a urine based FGFR3 mutation assay.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.