Article
Suppressor mutations in rpoA suggest that OmpR controls transcription by direct interaction with the alpha subunit of RNA polymerase.
Journal of bacteriology - 1 Dec 1991
Slauch J M, Russo F D, Silhavy T J
Abstract excerpt
We have isolated mutations in rpoA, the gene encoding the alpha subunit of RNA polymerase, that specifically affect transcriptional control by OmpR and EnvZ, the two-component regulatory system that controls porin gene expression in Escherichia coli. Characterization of these mutations and a prev...
Topics
- Alleles
- Bacterial Outer Membrane Proteins
- Base Sequence
- DNA-Directed RNA Polymerases
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Genes, Bacterial
- Genotype
- Macromolecular Substances
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Oligodeoxyribonucleotides
- Polymerase Chain Reaction
- Porins
- Recombinant Fusion Proteins
- Transcription, Genetic
- beta-Galactosidase