Article
Morphological and immunocytochemical analysis of Escherichia coli-specific surface antigens in wildtype strains and in recombinant Vibrio cholerae.
Antonie van Leeuwenhoek - 1 Jan 2000
Lüdi Stefan, Frey Joachim, Favre Didier, Viret Jean-François, Ziethlow Verena, Boghenbor Kathrin Kühni, Stoffel Michael H
Abstract excerpt
Adhesion is the first step in the pathogenesis of enterotoxigenic Escherichia coli infections. The genes encoding the most prevalent adhesion factors CFA/I, CS3 and CS6 were cloned into Vibrio cholerae strain CVD 103-HgR and expression of fimbriae was investigated in wildtype and recombinant strains by transmission electron microscopy in conjunction with immunolabelling and negative staining. Negative staining...
Topics
- Antigens, Bacterial
- DNA, Recombinant
- Escherichia coli
- Fimbriae, Bacterial
- Genotype
- Immunohistochemistry
- Microscopy, Electron, Transmission
- Vibrio cholerae
