Genotypic detection of enterotoxigenic Escherichia coli colonisation factors.

doi:10.1034/j.1600-0463.2001.090607.x

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Article

Genotypic detection of enterotoxigenic Escherichia coli colonisation factors.

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica - 1 Jun 2001

Grewal H M, Sommerfelt H

Abstract excerpt

We have developed a nonradioactive colony hybridisation assay for the detection of enterotoxigenic Escherichia coli (ETEC) that harbor the structural genes for CFA/I, CS1, CS2, CS4, CS17, or PCFO166. Thus, a polynucleotide probe derived from the colonisation factor antigen I (CFA/I) operon hybridised under very low stringency conditions to total DNA from CFA/I-producing (CFA/I), coli-surface antigen 1 and 3 (CS1...

Topics

Animals
Antigens, Bacterial
Bacterial Proteins
DNA Probes
DNA, Bacterial
Enterotoxins
Escherichia coli
Fimbriae Proteins
Genes, Bacterial
Genotype
Humans
Nucleic Acid Hybridization
Plasmids

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Open in PubMed

Identifiers

PMID: 11506477
DOI: 10.1034/j.1600-0463.2001.090607.x

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