Expression in Escherichia coli of fragments of the coiled-coil rod domain of rabbit myosin: influence of different regions of the molecule on aggregation and paracrystal formation.

Abstract excerpt

We have expressed in Escherichia coli a cDNA clone corresponding broadly to rabbit light meromyosin (LMM) together with a number of modified polypeptides and have used this material to investigate the role of different aspects of molecular structure on the solubility properties of LMM. The expres...

Topics

• Amino Acid Sequence
• Animals
• Base Sequence
• Crystallization
• Escherichia coli
• Hydrogen-Ion Concentration
• Microscopy, Electron
• Molecular Sequence Data
• Mutation
• Myosin Subfragments
• Osmolar Concentration
• Rabbits
• Recombinant Proteins
• Solubility