Article
Structure and property engineering of α-D-glucans synthesized by dextransucrase mutants.
Biomacromolecules - 9 Jan 2012
Irague Romain, Rolland-Sabaté Agnès, Tarquis Laurence, Doublier Jean Louis, Moulis Claire, Monsan Pierre, Remaud-Siméon Magali, Potocki-Véronèse Gabrielle, Buléon Alain
Abstract excerpt
Seven dextran types, displaying from 3 to 20% α(1→3) glycosidic linkages, were synthesized in vitro from sucrose by mutants of dextransucrase DSR-S from Leuconostoc mesenteroides NRRL B-512F, obtained by combinatorial engineering. The structural and physicochemical properties of these original biopolymers were characterized. When asymmetrical flow field flow fractionation coupled with multiangle laser light...
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