Transformation of the phytopathogenic bacterium Clavibacter michiganense subsp. michiganense by electroporation and development of a cloning vector.

doi:10.1128/jb.173.1.184-190.1991

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Article

Transformation of the phytopathogenic bacterium Clavibacter michiganense subsp. michiganense by electroporation and development of a cloning vector.

Journal of bacteriology - 1 Jan 1991

Meletzus D, Eichenlaub R

Abstract excerpt

We constructed a cloning vector for use in the plant pathogenic bacterium Clavibacter michiganense subsp. michiganense. The vector pDM100 consists of a 3.2-kb restriction fragment of the Clavibacter plasmid pCM1 joined to a pBR325 derivative carrying the neomycin phosphotransferase of transposon...

Topics

Actinomycetales
Cloning, Molecular
DNA, Bacterial
Electric Stimulation
Escherichia coli
Genetic Techniques
Genetic Vectors
Phenotype
Plasmids
Restriction Mapping
Transfection
Transformation, Bacterial

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