Article
MP3RNA-seq: Massively parallel 3' end RNA sequencing for high-throughput gene expression profiling and genotyping.
Journal of integrative plant biology - 1 Jul 2021
Chen Jian, Zhang Xiangbo, Yi Fei, Gao Xiang, Song Weibin, Zhao Haiming, Lai Jinsheng
Abstract excerpt
Transcriptome deep sequencing (RNA-seq) has become a routine method for global gene expression profiling. However, its application to large-scale experiments remains limited by cost and labor constraints. Here we describe a massively parallel 3' end RNA-seq (MP3RNA-seq) method that introduces unique sample barcodes during reverse transcription to permit sample pooling immediately following this initial step....
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