Article
Specific inhibition of caspase-8 and -9 in CHO cells enhances cell viability in batch and fed-batch cultures.
Metabolic engineering - 1 Jan 2000
Yun Chee Yong, Liu Sen, Lim Sing Fee, Wang Tianhua, Chung Beatrice Y F, Jiat Teo Joong, Chuan Kok Hwee, Soon Allyson S C, Goh Keng Siong, Song Zhiwei
Abstract excerpt
In an attempt to investigate the molecular mechanism that leads to apoptotic death in Chinese hamster ovary (CHO) cells in batch and fed-batch cultures, we cloned caspase-2, -8 and -9 from a CHO cDNA library. Recombinant Chinese hamster caspase-2 and -9 expressed in Escherichia coli show highest activities towards commercial peptide substrates Ac-VDVAD-pNA and Ac-LEHD-pNA, the designated commercial substrates for...
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