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Development of One-Step Reverse Transcriptase–Polymerase Chain Reaction to Detect Duck Hepatitis Virus Type 1

30 May 2007

Abstract excerpt

A one-step reverse transcriptase-polymerase chain reaction (RT-PCR) method was developed and optimized for the detection of duck hepatitis virus type 1 (DHV-1) using the Viral Gene-spin viral DNA/RNA extraction kit. A pair of DHV-1-specific primers was designed against the gene encoding RNA-dependent RNA polymerase (3D gene). Using RNA prepared from duckling liver samples infected with two reference and seven...

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PMID: 17626480
DOI: 10.1637/0005-2086(2007)51[540:doortc]2.0.co;2

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