Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Development of a One-Step Duplex RT-PCR Method for the Simultaneous Detection of VP3/VP1 and VP1/P2B Regions of the Hepatitis A Virus.

Journal of microbiology and biotechnology - 28 Aug 2016

Kim Mi-Ju, Lee Shin-Young, Kim Hyun-Joong, Lee Jeong Su, Joo In Sun, Kwak Hyo Sun, Kim Hae-Yeong

Abstract excerpt

The simultaneous detection and accurate identification of hepatitis A virus (HAV) is critical in food safety and epidemiological studies to prevent the spread of HAV outbreaks. Towards this goal, a one-step duplex reverse-transcription (RT)-PCR method was developed targeting the VP1/P2B and VP3/VP1 regions of the HAV genome for the qualitative detection of HAV. An HAV RT-qPCR standard curve was produced for the...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Capsid Proteins
Share to Food Microbiology

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Genome, Viral

Share to Hepatitis A virus

Share to Limit of Detection

Share to Peptide Fragments

Share to RNA, Viral

Share to Viral Structural Proteins

Source

Identifiers

PMID: 27197668
DOI: 10.4014/jmb.1604.04047

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Development of a One-Step Duplex RT-PCR Method for the Simultaneous Detection of VP3/VP1 and VP1/P2B Regions of the Hepatitis A Virus.PMID 27197668Read ↗

Whole genome sequencing-based confirmatory methods on RT-qPCR results for detection of foodborne viruses in frozen berriesSemantic proximity Read ↗

Development, Evaluation, and Standardization of a Real-Time TaqMan Reverse Transcription-PCR Assay for Quantification of Hepatitis A Virus in Clinical and Shellfish SamplesSemantic proximity Read ↗

A new assay for quantitative detection of hepatitis A virusSemantic proximity Read ↗

Simple and feasible detection of hepatitis a virus using reverse transcription multienzyme isothermal rapid amplification and lateral flow dipsticks without standard PCR laboratorySemantic proximity Read ↗

Comparison of sample pretreatments used to distinguish between infectious and non-infectious foodborne viruses by RT-qPCRSemantic proximity Read ↗

Development and application of a one-step real-time Taqman RT-PCR assay for detection of Duck hepatitis virus type1Semantic proximity Read ↗

Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCRSemantic proximity Read ↗

One-step duplex RT-droplet digital PCR assay for the detection of norovirus GI and GII in lettuce and strawberry.Semantic proximity Read ↗

Development and Evaluation of a Molecular Hepatitis A Virus Assay for Serum and Stool Specimens.Semantic proximity Read ↗

Detecting the presence of infectious hepatitis A virus in molluscs positive to RT-nested-PCRSemantic proximity Read ↗

A one-step duplex rRT-PCR assay for the simultaneous detection of duck hepatitis A virus genotypes 1 and 3.Semantic proximity Read ↗

Multiplex Nucleic Acid Sequence-Based Amplification for Simultaneous Detection of Several Enteric Viruses in Model Ready-To-Eat FoodsSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.