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Article

Simple, specific molecular typing of dengue virus isolates using one-step RT-PCR and restriction fragment length polymorphism.

Journal of virological methods - 1 Oct 2012

Ortiz Alma, Capitan Zeuz, Mendoza Yaxelis, Cisneros Julio, Moreno Brechla, Zaldivar Yamitzel, Garcia Mariana, Smith Rebecca E, Motta Jorge, Pascale Juan Miguel

Abstract excerpt

A one-step RT-PCR and one-enzyme RFLP was used to detect and distinguish among flaviviruses, including the four serotypes of dengue and the St. Louis Encephalitis, West Nile and Yellow Fever viruses in cultured virus samples or acute-phase human serum. Using a previously described RT-PCR, but novel RFLP procedure, results are obtained in 24 h with basic PCR and electrophoresis equipment. There is 95% agreement...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 22766181
DOI: 10.1016/j.jviromet.2012.06.016

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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