<scp>D</scp>‐Mannitol production by resting state whole cell biotransformation of <scp>D</scp>‐fructose by heterologous mannitol and formate dehydrogenase gene expression in <i>Bacillus megaterium</i>

Abstract excerpt

An in vivo system was developed for the biotransformation of D-fructose into D-mannitol by the expression of the gene mdh encoding mannitol dehydrogenase (MDH) from Leuconostoc pseudomesenteroides ATCC12291 in Bacillus megaterium. The NADH reduction equivalents necessary for MDH activity were regenerated via the oxidation of formate to carbon dioxide by coexpression of the gene fdh encoding Mycobacterium vaccae...

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