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Cloning, Nucleotide Sequence, and Overexpression of the <scp>l</scp> -Rhamnose Isomerase Gene from <i>Pseudomonas stutzeri</i> in <i>Escherichia coli</i>

1 Jun 2004

Abstract excerpt

The gene encoding L-rhamnose isomerase (L-RhI) from Pseudomonas stutzeri was cloned into Escherichia coli and sequenced. A sequence analysis of the DNA responsible for the L-RhI gene revealed an open reading frame of 1,290 bp coding for a protein of 430 amino acid residues with a predicted molecular mass of 46,946 Da. A comparison of the deduced amino acid sequence with sequences in relevant databases indicated...

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Identifiers

PMID: 15184124
DOI: 10.1128/aem.70.6.3298-3304.2004

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