Article
High phosphatidylinositol 4-phosphate (PI4P)-dependent ATPase activity for the Drs2p-Cdc50p flippase after removal of its N- and C-terminal extensions.
The Journal of biological chemistry - 12 May 2017
Azouaoui Hassina, Montigny Cédric, Dieudonné Thibaud, Champeil Philippe, Jacquot Aurore, Vázquez-Ibar José Luis, Le Maréchal Pierre, Ulstrup Jakob, Ash Miriam-Rose, Lyons Joseph A, Nissen Poul, Lenoir Guillaume
Abstract excerpt
P4-ATPases, also known as phospholipid flippases, are responsible for creating and maintaining transbilayer lipid asymmetry in eukaryotic cell membranes. Here, we use limited proteolysis to investigate the role of the N and C termini in ATP hydrolysis and auto-inhibition of the yeast flippase Drs2p-Cdc50p. We show that limited proteolysis of the detergent-solubilized and purified yeast flippase may result in more...
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