Article
Isolation of arginine auxotrophs, cloning by mutant complementation, and sequence analysis of the argC gene from the cyanobacterium Anabaena species PCC 7120.
Molecular microbiology - 1 Aug 1992
Floriano B, Herrero A, Flores E
Abstract excerpt
Arginine auxotrophs of the dinitrogen-fixing cyanobacterium Anabaena species strain PCC 7120 were isolated after ultraviolet light mutagenesis and penicillin enrichment. Two of these auxotrophs were complemented by a cosmid gene library of the wild-type strain established in Escherichia coli that was transferred en masse to the mutants by conjugation. The gene complementing one of those mutants was found to...
Topics
- Aldehyde Oxidoreductases
- Amino Acid Sequence
- Anabaena
- Arginine
- Bacterial Proteins
- Base Sequence
- Cloning, Molecular
- Genes, Bacterial
- Genetic Complementation Test
- Molecular Sequence Data
- Mutation