Article
Influence of the cell wall on intracellular delivery to algal cells by electroporation and sonication.
Ultrasound in medicine & biology - 1 Nov 2007
Azencott Harold R, Peter Gary F, Prausnitz Mark R
Abstract excerpt
To assess the cell wall's role as a barrier to intracellular delivery, wild-type Chlamydomonas reinhardtii algal cells and mutant cells lacking a cell wall were exposed to electroporation or sonication. Flow cytometry determined intracellular uptake of calcein and bovine serum albumin (BSA) and loss of cell viability as functions of electroporation transmembrane potential and acoustic energy. Electroporation of...
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