Article
Evaluation of intracellular lipid bodies in Chlamydomonas reinhardtii strains by flow cytometry.
Bioresource technology - 1 Jun 2013
Velmurugan Natarajan, Sung Minji, Yim Sung Sun, Park Min S, Yang Ji Won, Jeong Ki Jun
Abstract excerpt
A comparative study of Chlamydomonas reinhardtii wild type CC124 and a cell wall-less mutant sta6-1 is described using FACS in conjunction with two different lipophilic fluorescent dyes, Nile Red and BODIPY 505/515. The results indicate that BODIPY 505/515 is more effective for the vital staining of intracellular lipid bodies and single cell sorting than Nile Red. While BODIPY 505/515 stained cells continued to...
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