Article
Efficient gene identification and targeted gene disruption in the wheat blotch fungus Mycosphaerella graminicola using TAGKO.
Current genetics - 1 Nov 2002
Adachi Kiichi, Nelson Grant H, Peoples Keith A, Frank Sheryl A, Montenegro-Chamorro Maria V, DeZwaan Todd M, Ramamurthy Lakshman, Shuster Jeffrey R, Hamer Lisbeth, Tanzer Matthew M
Abstract excerpt
TAGKO ( transposon- arrayed gene knock out) is a highly efficient method for gene discovery and gene function assignment in the rice blast fungus Magnaporthe grisea. Here, we report the application of genome-wide TAGKO to the wheat blotch fungus Mycosphaerella graminicola, including the successful development of electroporation-based transformation for this fungus. A M. graminicola genomic cosmid library was...
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