Article
'Crystal lattice engineering,' an approach to engineer protein crystal contacts by creating intermolecular symmetry: crystallization and structure determination of a mutant human RNase 1 with a hydrophobic interface of leucines.
Protein science : a publication of the Protein Society - 1 Jul 2007
Yamada Hidenori, Tamada Taro, Kosaka Megumi, Miyata Kohei, Fujiki Shinya, Tano Masaru, Moriya Masayuki, Yamanishi Mamoru, Honjo Eijiro, Tada Hiroko, Ino Takeshi, Yamaguchi Hiroshi, Futami Junichiro, Seno Masaharu, Nomoto Takashi, Hirata Tomoko, Yoshimura Motonobu, Kuroki Ryota
Abstract excerpt
A protein crystal lattice consists of surface contact regions, where the interactions of specific groups play a key role in stabilizing the regular arrangement of the protein molecules. In an attempt to control protein incorporation in a crystal lattice, a leucine zipper-like hydrophobic interface (comprising four leucine residues) was introduced into a helical region (helix 2) of the human pancreatic...
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