Article
Time- and temperature-dependent acetylation of the chemokine RANTES produced in recombinant Escherichia coli.
Protein expression and purification - 1 Sept 2007
d'Alayer Jacques, Expert-Bezançon Nicole, Béguin Pierre
Abstract excerpt
The S24F mutant of the chemokine RANTES was found to be partly acetylated when produced in recombinant Escherichia coli BL21(DE3)(pDIA17)(CCL5-S24F-pET-26b). Mass spectrometry and Edman sequencing of peptides generated by lys-C endopeptidase indicated that Lys-26, Lys-34, Lys-46, and Lys-57 were susceptible to acetylation. The extent of acetylation of the RANTES S24F polypeptide increased with temperature and...
Topics
- Acetyl Coenzyme A
- Acetylation
- Acetyltransferases
- Amino Acid Sequence
- Chemokine CCL5
- Chemokines
- Cold Temperature
- Escherichia coli
- Escherichia coli Proteins
- Gene Deletion
- Humans
- Lysine
- Molecular Sequence Data
- Mutation
- Recombinant Proteins
- Sequence Analysis, Protein
- Temperature
- Time Factors