Article
Isolation and characterization of mutated alcohol oxidases from the yeast Hansenula polymorpha with decreased affinity toward substrates and their use as selective elements of an amperometric biosensor.
BMC biotechnology - 13 Jun 2007
Dmytruk Kostyantyn V, Smutok Oleh V, Ryabova Olena B, Gayda Galyna Z, Sibirny Volodymyr A, Schuhmann Wolfgang, Gonchar Mykhailo V, Sibirny Andriy A
Abstract excerpt
BACKGROUND: Accurate, rapid, and economic on-line analysis of ethanol is very desirable. However, available biosensors achieve saturation at very low ethanol concentrations and thus demand the time and labour consuming procedure of sample dilution. RESULTS: Hansenula polymorpha (Pichia angusta) mutant strains resistant to allyl alcohol in methanol medium were selected. Such strains possessed decreased affinity of...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.