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Article

Microbial O2- and H2O2-electrode sensors for alcohol assays based on the use of permeabilized mutant yeast cells as the sensitive bioelements.

Biosensors & bioelectronics - 15 Oct 1998

Gonchar M V, Maidan M M, Moroz O M, Woodward J R, Sibirny A A

Abstract excerpt

Two types of alcohol-specific microbial/electrochemical biosensors have been developed using specially constructed mutant cells of the methylotrophic yeast Hansenula polymorpha. The cells were immobilized in a calcium alginate gel, and placed between two membranes on the surface of oxygen or hydrogen peroxide-electrodes. The O2 electrode based biosensor contained mutant cells with strongly elevated alcohol...

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PMID: 9839383
DOI: 10.1016/s0956-5663(98)00034-7

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