Article
[Attenuation of a genotype VIId Newcastle disease virus ZJI strain of goose origin by reverse genetics].
Wei sheng wu xue bao = Acta microbiologica Sinica - 1 Apr 2007
Hu Shun-lin, Sun Qing, Wu Yan-tao, Zhang Yan-mei, Liu Xiu-fan
Abstract excerpt
Based on the complete genome sequence of Newcastle disease virus (NDV) ZJI strain isolated from an outbreak in the goose, seven pairs of primers were designed to amplify cDNA fragment for constructing the plasmid pNDV/ZJI, which contained the full-length cDNA of NDV ZJI strain. The pNDV/ZJI with three helper plasmids, pCI-NP, pCI-P and pCI-L, were then cotransfected into BSR-T7/5 cells expressing T7 RNA...
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