Article
[Analysis of leader and trailer sequence of genotype III, VIb and VIId Newcastle disease virus determined by modified rapid amplification of cDNA ends (RACE) strategy].
Wei sheng wu xue bao = Acta microbiologica Sinica - 1 Jul 2009
Qiu Xusheng, Sun Qing, Wang Weiwei, Dong Li, Wu Shuang, Hu Shunling, Wu Yantao, Liu Xiufan
Abstract excerpt
OBJECTIVE: The purpose of this research is to establish a simple rapid amplification of cDNA ends (RACE) strategy for direct mapping of the 3' end and 5' end of the genomic RNA of Newcastle disease virus (NDV), and to analyze the leader and trailer sequence of NDV strains belonging to different genotypes. METHODS: Classic RNA Ligase Mediated Rapid Amplification of cDNA Ends (RLM-RACE) was specifically modified...
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