Article
Rapid Generation of a Recombinant Genotype VIII Newcastle Disease Virus (NDV) Using Full-Length Synthetic cDNA.
Current microbiology - 1 Apr 2021
Murulitharan Kavitha, Yusoff Khatijah, Omar Abdul Rahman, Peeters Ben P H, Molouki Aidin
Abstract excerpt
Rescue of (-)ssRNA viruses involves the sequential assembly and cloning of the full-length cDNA, which is often a challenging and time-consuming process. The objective of this study was to develop a novel method to rapidly clone the full-length cDNA of a very virulent NDV by only one assembly step. A completely synthetic 15 kb cDNA of a Malaysian genotype VIII NDV known as strain AF2240-I with additional flanking...
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