Article
Evaluation of an automated, highly sensitive, real-time PCR-based assay (COBAS Ampliprep/COBAS TaqMan) for quantification of HCV RNA.
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology - 1 Oct 2008
Sarrazin Christoph, Dragan Anette, Gärtner Barbara C, Forman Michael S, Traver Stella, Zeuzem Stefan, Valsamakis Alexandra
Abstract excerpt
BACKGROUND: Diagnosis of hepatitis C virus (HCV) infection and its therapy is based on qualitative and quantitative measurement of HCV RNA. OBJECTIVES: A new assay that employs automated specimen extraction and real-time RT-PCR (COBAS Ampliprep/COBAS TaqMan, "CAP/CTM", Roche Diagnostics, Pleasanton, USA) was designed for linear quantification and highly sensitive detection of HCV RNA. STUDY DESIGN: The...
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