Naturally occurring and bioengineered apoA-I mutations that inhibit the conversion of discoidal to spherical HDL: the abnormal HDL phenotypes can be corrected by treatment with LCAT.

Abstract excerpt

In the present study we have used adenovirus-mediated gene transfer of apoA-I (apolipoprotein A-I) mutants in apoA-I-/- mice to investigate how structural mutations in apoA-I affect the biogenesis and the plasma levels of HDL (high-density lipoprotein). The natural mutants apoA-I(R151C)Paris, apoA-I(R160L)Oslo and the bioengineered mutant apoA-I(R149A) were secreted efficiently from cells in culture. Their...

Topics

• ATP Binding Cassette Transporter 1
• ATP-Binding Cassette Transporters
• Adenoviridae
• Animals
• Apolipoprotein A-I
• Biological Transport
• Cell Line
• Cholesterol
• Chromatography, Liquid
• Enzyme Activation