Article
Role of a conserved salt bridge between the PAS core and the N-terminal domain in the activation of the photoreceptor photoactive yellow protein.
Biophysical journal - 1 Sept 2007
Hoersch Daniel, Otto Harald, Joshi Chandra P, Borucki Berthold, Cusanovich Michael A, Heyn Maarten P
Abstract excerpt
The effect of ionic strength on the conformational equilibrium between the I(2) intermediate and the signaling state I(2)' of the photoreceptor PYP and on the rate of recovery to the dark state were investigated by time-resolved absorption and fluorescence spectroscopy. With increasing salt concentration up to approximately 600 mM, the recovery rate k(3) decreases and the I(2)/I(2)' equilibrium (K) shifts in the...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
