Article
Complementation of an E. coli cysteine auxotrophic mutant for the structural modification study of 3'(2'),5'-bisphosphate nucleotidase.
Biotechnology letters - 1 Jun 2007
Cheong Jong-Joo, Hwang Ingyu, Rhee Sangkee, Moon Tae Wha, Choi Yang Do, Kwon Hawk-Bin
Abstract excerpt
The Arabidopsis AHL gene encodes a 3'(2'),5'-bisphosphate nucleotidase (BPNTase) involved in the reductive sulfate activation pathway. A bacterial expression vector containing AHL cDNA was randomly mutagenized with hydroxylamine and transformed into the E. coli cysteine auxotrophic mutant cysQ. Bacterial colonies that did not show evidence of complementation, i.e. those that exhibited slower growth on...
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