Article
Herbicide-resistant forms of Arabidopsis thaliana acetohydroxyacid synthase: characterization of the catalytic properties and sensitivity to inhibitors of four defined mutants.
The Biochemical journal - 1 Aug 1998
Chang A K, Duggleby R G
Abstract excerpt
Acetohydroxyacid synthase (AHAS) catalyses the first step in the synthesis of the branched-chain amino acids and is the target of several classes of herbicides. Four mutants (A122V, W574S, W574L and S653N) of the AHAS gene from Arabidopsis thaliana were constructed, expressed in Escherichia coli,...
Topics
- Acetolactate Synthase
- Arabidopsis
- Catalysis
- Enzyme Activation
- Enzyme Inhibitors
- Enzyme Stability
- Flavin-Adenine Dinucleotide
- Herbicides
- Hydrogen-Ion Concentration
- Kinetics
- Magnesium
- Mutation
- Pyruvic Acid
- Sensitivity and Specificity
- Substrate Specificity
- Thiamine Pyrophosphate