Article
The role of the membrane domain in the regulated degradation of 3-hydroxy-3-methylglutaryl coenzyme A reductase.
The Journal of biological chemistry - 25 Feb 1992
Chun K T, Simoni R D
Abstract excerpt
We have constructed a series of mutations in the membrane and linker domains of Syrian hamster 3-hydroxy-3-methylglutaryl-(HMG) CoA reductase in order to determine the regions critical for the regulated degradation of the enzyme. In transfected Chinese hamster ovary cells, we have expressed a fus...
Topics
- Amino Acid Sequence
- Animals
- CHO Cells
- Cell Membrane
- Cricetinae
- Gene Expression
- Hydroxycholesterols
- Hydroxymethylglutaryl CoA Reductases
- Lipoproteins, LDL
- Mevalonic Acid
- Molecular Sequence Data
- Mutation
- beta-Galactosidase