Article
Human cytoplasmic 3-hydroxy-3-methylglutaryl coenzyme A synthase: expression, purification, and characterization of recombinant wild-type and Cys129 mutant enzymes.
Archives of biochemistry and biophysics - 1 Jul 1994
Rokosz L L, Boulton D A, Butkiewicz E A, Sanyal G, Cueto M A, Lachance P A, Hermes J D
Abstract excerpt
A cDNA for the human cytoplasmic 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase (EC 4.1.3.5) was subcloned and expressed from a T7-based vector in Escherichia coli. The over-produced enzyme was purified using a three-step protocol that generated 20 to 30 mg protein/liter cell culture. T...
Topics
- Acetyl Coenzyme A
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- Cysteine
- Escherichia coli
- Fatty Acids, Unsaturated
- Fluorescent Dyes
- Gene Library
- Humans
- Hydroxymethylglutaryl-CoA Synthase
- Lactones
- Molecular Sequence Data
- Mutagenesis, Site-Directed