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Article

Transition-state characterization: a new approach combining inhibitor analogues and variation in enzyme structure.

Biochemistry - 4 Feb 1992

Phillips M A, Kaplan A P, Rutter W J, Bartlett P A

Abstract excerpt

A new strategy of potentially broad application for probing transition-state (TS) analogy in enzymatic systems is described in this paper. The degree to which a series of phosphonate inhibitors act as TS analogues of rat carboxypeptidase A1 has been determined for the wild-type enzyme, for the R127K, R127M, and R127A mutants, and for the R127A mutant in the presence of 0.5 M guanidine hydrochloride. The impact...

Topics

Animals
Carboxypeptidases
Carboxypeptidases A
Catalysis
Dipeptides
Genetic Variation
Guanidine
Guanidines
Hydrolysis
Kinetics
Mutation
Organophosphonates

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Rats

Structure-Activity Relationship

Substrate Specificity

Source

Identifiers

PMID: 1734971
DOI: 10.1021/bi00119a003

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1734971-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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