Article
Guanidine derivatives restore activity to carboxypeptidase lacking arginine-127.
Protein science : a publication of the Protein Society - 1 Apr 1992
Phillips M A, Hedstrom L, Rutter W J
Abstract excerpt
Arg-127 stabilizes the oxyanion of the tetrahedral intermediate formed during Zn2+ carboxypeptidase A-catalyzed hydrolysis. Mutant carboxypeptidases lacking Arg-127 exhibit substantially reduced rates of hydrolysis with the change manifest almost entirely in kcat (kcat/Km is decreased by 10(4) fo...
Topics
- Animals
- Arginine
- Carboxypeptidases
- Carboxypeptidases A
- Dipeptides
- Guanidine
- Guanidines
- Methylguanidine
- Mutation
- Peptides
- Rats
- Substrate Specificity