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Article

Computer-aided design of the stability of pyruvate formate-lyase from Escherichia coli by site-directed mutagenesis.

Bioscience, biotechnology, and biochemistry - 1 Mar 2007

Yang Deng-Feng, Wei Yu-Tuo, Huang Ri-Bo

Abstract excerpt

Using computer-aided design of single-site mutations, three amino acid residues determined by changes in folding free energy between wild-type (wt) and mutant proteins were exchanged to enhance the stability of pyruvate formate-lyase (PFL). The mutant enzymes were tested for properties such as op...

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Identifiers

PMID: 17341838
DOI: 10.1271/bbb.60576

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-17341838-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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