Article
EGF-R antisense RNA blocks expression of the epidermal growth factor receptor and suppresses the transforming phenotype of a human carcinoma cell line.
The Journal of biological chemistry - 5 Feb 1992
Moroni M C, Willingham M C, Beguinot L
Abstract excerpt
We have used an antisense approach to investigate the role of overexpression of the normal human epidermal growth factor (EGF) receptor in the transformed phenotype of KB cells, which are a tumor derived human cell line. Initial experiments performed in vitro, showed that antisense RNA complement...
Topics
- Carcinoma, Squamous Cell
- Cell Transformation, Neoplastic
- Culture Media, Serum-Free
- Epidermal Growth Factor
- ErbB Receptors
- Gene Expression
- Humans
- Microscopy, Fluorescence
- Phenotype
- Plasmids
- Precipitin Tests
- Protein Biosynthesis
- RNA, Antisense
- RNA, Messenger
- Transcription, Genetic
- Transfection
- Tumor Cells, Cultured