Article
Absence of reciprocal feedback between MPF and ERK2 MAP kinase in mitotic Xenopus laevis embryo cell-free extract.
Cell cycle (Georgetown, Tex.) - 15 Feb 2007
Bazile Franck, Pascal Aude, Karaiskou Anthi, Chesnel Franck, Kubiak Jacek Z
Abstract excerpt
MPF and MAP kinase ERK2 are two major M-phase kinases. They interact with each other in a complex way during meiotic maturation of Xenopus laevis oocytes. Here we study their interrelationship during first mitosis in X. laevis embryo cell-free extract perturbing the polyubiquitination pathway as a tool. Recombinant ubiquitin K48R (Ub-K48R) mutant protein arrests mitotic cyclin B degradation in the extract. This...
Topics
- Animals
- Butadienes
- Cell-Free System
- Embryo, Nonmammalian
- Feedback, Physiological
- Female
- Leupeptins
- Maturation-Promoting Factor
- Meiosis
- Mitogen-Activated Protein Kinase 1
- Mitosis
- Mutation
- Nitriles
- Phosphorylation